Virginia Key in Miami-Dade County, Florida, USA, is an island lying in Biscayne Bay between Key Biscayne and Miami. It is connected to the mainland at Miami by the Rickenbacker Causeway toll road and is the site of a large sewage treatment plant and a former landfill for the City of Miami.

The southern end of Virginia Key is also home to the former Virginia Key Park (for use by African-Americans when other parks were reserved for 'whites-only'), Miami Seaquarium, the University of Miami’s Rosenstiel School of Marine Science, the National Marine Fisheries Service’s Southeast Fisheries Science Center, the U.S. National Oceanic and Atmospheric Administration’s Atlantic Oceanographic and Meteorological Laboratory, "Hobie Beach", and several restaurants.

The land area of the island is 3.493 km² (1.349 sq mi, or 863.24 acres). There is no permanent resident population, except maybe at the traditional and famous "Jimbo's" where some locals do reside.